Verification of the utility of molecular markers linked to the multiple-allele male-sterile gene Ms in the breeding of male-sterile lines of Chinese cabbage (Brassica rapa)

  • Feng Hui
  • Wang Lili
  • Wei Peng
  • Liu Zhiyong
  • Li Chengyu
  • Wang Yugang
  • Ji Ruiqin
  • Zhang Huaqing

Abstract

To verify the molecular markers linked to the genic multiple-allele male-sterile gene Ms, an F1 plant, which was generated by crossing the inbred line a20 and the male-sterile plant of the genic multipleallele male-sterile AB line, was backcrossed with an a20 plant to develop BC4 and BC5 populations. Sequence-characterized amplified region (SCAR) marker syau_scr01 and simple sequence repeat (SSR) marker syau_m13, which were linked to Ms, exhibited polymorphism between the 2 parents. The accuracies of these 2 markers in determining the plant genotype was 85 and 91.7%, respectively. The accuracy reached 100% when the 2 markers were used in combination. These results indicate that these 2 markers can be applied in the marker-assisted selection of the genic multiple-allele male-sterile line of Chinese cabbage.

Keywords: Chinese cabbage, genic multiple-allele male sterility, marker-assisted selection, simple sequence repeat, sequence-characterized amplified region

African Journal of Biotechnology Vol. 9(35), pp. 5623-5628, 30 August, 2010

Author Biographies

Feng Hui
Department of Horticulture, Shenyang Agricultural University 110161, China
Wang Lili
Department of Horticulture, Shenyang Agricultural University 110161, China
Wei Peng
Department of Horticulture, Shenyang Agricultural University 110161, China
Liu Zhiyong
Department of Horticulture, Shenyang Agricultural University 110161, China
Li Chengyu
Department of Horticulture, Shenyang Agricultural University 110161, China
Wang Yugang
Department of Horticulture, Shenyang Agricultural University 110161, China
Ji Ruiqin
Department of Horticulture, Shenyang Agricultural University 110161, China
Zhang Huaqing
Department of Horticulture, Shenyang Agricultural University 110161, China
Published
2013-08-16
Section
Articles

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