Comparative analysis of transformed potato microtubers and its non-transformed counterpart using some biochemical analysis along with inter simple sequence repeat (ISSR) marker
The rapid progress of transgenic biotechnology has significantly promoted the development and production of genetically modified (GM) crops. The aim of this study was to compare some compositional analysis and genetic variation of transformed potato microtubers (Solanum tuberosum L. Desiree) line (which harbor potato virus Y coat protein gene) with its conventional line in order to understand the metabolic and genomic changes associated with the transformation process. The constituents measured were: amino acids profile, minerals content, reducing sugar, vitamin C, total phenolic, total flavonoid, β-carotene and antioxidant activity. Also, PCR based assay using inter simple sequence repeat (ISSR) was employed to characterize the genetic variation between both lines. The data demonstrated that, there were no significant differences between the transformed and nontransformed line in total amount of amino acids, some minerals, total phenolic, total flavonoid and β- carotene contents. Obtained results of the ISSR profile was efficiently discriminated in both selected lines at DNA level. Generally, it was concluded that the transformed microtubers line was confirmed to be similar to that of the non-transformed microtubers line.
Key words: Amino acids, genetic modification, phenolic content, reducing sugar, Solanum tuberosum.