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High-density cultivation of <i>Lactobacillus plantarum</i> NCU116 in an ammonium and glucose fed-batch system


T Xiong
X Huang
J Huang
S Song
C Feng
M Xie

Abstract

Lactic acid bacteria (LAB) constitute a heterogeneous group of bacteria which are found in diverse environments such as the human body, animals and plants. In this study, we reported a simple fedbatch process for Lactobacillus plantarum NCU116 with high cell density. It was found that the optimum initial glucose concentration for this strain was 5% (w/v). To reduce the effect of acid and starvation, the exponential fed-batch culture and ammonium fed-batch system were introduced. At the end of the exponential phase of fermentation, the cell concentration was up to 9.35 × 109 CFU/ml.

Key words: Lactobacillus plantarum, high cell density cultivation, exponential fed-batch, ammonium.


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eISSN: 1684-5315