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African Journal of Biotechnology

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Generation and analysis of cDNA library from lipopolysaccharide-stimulated gastropod abalone (Haliotis diversicolor supertexta) hemocytes

L Wu, L Wang, X Wu

Abstract


A cDNA Library of lipopolysaccharide-stimulated gastropod abalone hemocytes was constructed using switching mechanism at 5' end of RNA transcript (SMART) approach. 300 random clones were selected and sequenced. 180 ESTs was gained. After assembling, we obtained 24 contigs and 119 singlets finally, some of which were immune- related genes. These immune-related genes include cytidine deaminase, ferritin, nonmuscle myosin essential light chain, cytochrome c oxidase subunit I, CD63 antigen-like protein and lysosomal-associated transmembrane protein. This study may contribute to the understanding of the immune mechanism of gastropod abalone Haliotis diversicolor supertexta.

Key words: Haliotis diversicolor supertexta, cDNA Library, gene expression, immune mechanism.




http://dx.doi.org/10.5897/AJB11.478
AJOL African Journals Online