African Journal of Biotechnology

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Differentiation studies of predominant lactic acid bacteria isolated during growol fermentation by using polyphasic taxonomic characterization

WDW Putri, M Mika, Y Nakagawa, H Kawasaki, NM Cahyanto, DW Marseno


Twelve isolates known as weakly amylolytic lactic acid bacteria were isolated from different time during growol fermentation, a cassava based product from Indonesia. Differentiation tests of these strains were performed using molecular and phenotypic characterization. 16S subunit of the ribosomal RNA and phenylalanyl tRNA synthase (pheS) gene sequences of all isolates showed similarity with Lactobacillus plantarum subsp. plantarum NBRC 15891 as a reference strain. But many isolates revealed different pattern of template DNA and carbohydrate fermentation profile which determined employing random amplified polymorphic DNA (RAPD) and API 50 CHL analysis, respectively. This result allowed three groups of isolates belonging to the species L. plantarum, to be distinguished. The comparative methods of identification showed considerable genome variation between lactic acid bacteria isolated from different time of fermentation, thus, becoming useful information for using the isolates as starter culture.

Key words: Growol, lactic acid bacteria, polyphasic taxonomic.
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