Subcelluar localization of orf126 of Bombyx mori nucleopolyhedrovirus
In order to explore the mechanism of orf126 of Bombyx mori nucleopolyhedro virus, the subcellular localization of ORF126 was conducted. The egfp gene was fused with the C-terminal of orf126 genes, BmN cells were transfected with different plasmid DNA and the superinfection were performed at 12 h post transfection. The fluorescence was examined by confocal laser scanning microscopy at different time point after transfection. The results show that EGFP protein was uniformly present throughout the cytoplasm and nucleus either in expression alone or superinfection, however, the fluorescence of EGFP linked to ORF126s were present barely in the cytoplasm.
Key words: BmNPV, orf126, transient expression, subcellular localization.