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African Journal of Biotechnology

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Genetic analysis of wild apple resources in Shandong province based on inter-simple sequence repeats (ISSR) and sequence-specific amplification polymorphism (S-SAP) markers

P He, L Li, H Li, H Wang, J Yang, Y Wang

Abstract


Apple (malus domestica Borkh.), which is a widely cultivated, important and economic fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop in this post-genomic era. Wild apple resources are important and they develop gradually in apple industry and genetic diversity. In this study, two molecular markers (inter-simple sequence repeats, ISSR and sequence-specific amplification polymorphism, S-SAP) were evaluated for genetic analysis of 31 wild apple resources in Shandong. A total of 20 ISSR primers were used and 110 polymorphic bands were amplified. Five S-SAP primer sets yielded a total of 496 bands, of which 201 (40.52%) were polymorphic. The similarity coefficient between resources ranged from 0.70 to 0.94 for ISSR analysis and from 0.66 to 0.95 using the S-SAP methodology. This study indicates that the results obtained based on the dendrograms constructed using unweighted pair-group using arithmetic average (UPGMA) cluster analysis were significantly correlated. The ISSR and S-SAP markers were found to be useful for wild apple resources identification and assessment of phonetic relationships.

Key words: Apple, wild resource, inter-simple sequence repeats (ISSR), sequence-specific amplification polymorphism (S-SAP).




http://dx.doi.org/10.5897/AJB11.1111
AJOL African Journals Online