PROMOTING ACCESS TO AFRICAN RESEARCH

African Journal of Biotechnology

Log in or Register to get access to full text downloads.

Remember me or Register



Analysis of the effects of cerium on calcium ion in the protoplasts of Arabidopsis thaliana with confocal microscopy

D Liu, Z Wang, Y Lin, L Sun, Z Chen, H Xu

Abstract


The laser-scanning confocal microscopy has become a routine technique and indispensable tool for cell biological studies. In this study, the probe Fluo-3 AM was used to research the instantaneous changes of calcium ion (Ca2+) in the protoplasts of Arabidopsis thaliana. The laser-scanning mode of confocal microscope is XYT, and the time interval between two images is 10.635 s. 30 optical sections were acquired with the laser-scanning confocal microscope, and the different concentrations of trivalent cerium (Ce3+) (0.1, 0.5 and 1.0 mmol/L) were added after four optical sections were scanned, respectively. Furthermore, the fluorescence intensity of Ca2+ in the 30 optical sections was quantified with Leica Confocal Software. The quantitative data was exported and the trend chart was made with Excel software. The results indicate that Ca2+ concentration in the protoplasts of A. thaliana declined after 0.5 and 1.0 mmol/L Ce3+ was added. However, Ca2+ concentration slightly increased in the protoplasts treated with 0.1 mmol/L Ce3+. The data indicate that the appropriate amount of Ce3+ can inhibit intracellular Ca2+ concentration in the protoplasts of A. thaliana. Since Ca2+ participates in signal transduction and plays a crucial role in regulating plant growth and development under environmental stresses, Ce3+ may regulate plant metabolism, growth and development via the changes of Ca2+ concentration.

Key words: Cerium, calcium ion, protoplast, confocal microscope.




http://dx.doi.org/10.5897/AJB11.946
AJOL African Journals Online