Plant regeneration system from cotyledons-derived calluses cultures of <i>Stylosanthes guianensis</i> cv. ‘Reyan 2’

Abstract

The objective of this study was to successfully establish plant regeneration system with cotyledons of Stylosanthes guianensis Sw. cv. ‘Reyan 2’ as explants. In this study, the following results were obtained; (1) the highest rates of callus induction on medium MS with 3.0 mg L^-1 2, 4-D with cotyledons as explants were 74%. The above medium of formulation was adopted for subculture; (2) the highest rates of callus differentiation medium MS with 3.0 mg L^-1 6-BA with cotyledons as explants was 66%; (3) the optimum medium of shoot growth was MS medium plus 1.0 mg L^-1 6-BA, 0.5 mg L^-1 NAA and 0.4 mg L^-1 GA; (4) the rooting rate was 60% on optimum rooting medium with 0.2 mg L^-1 NAA; (5) the survival rate of plant transplanting was 70% after transferred to pots with garden soil. In conclusion, the efficient plant regeneration system developed here will be helpful for rapid micropropagation and further genetic improvement in S. guianensis Sw. cv. ‘Reyan 2’.

Key words: Stylosanthes guianensis Sw. cv. Reyan 2, callus, cotyledons, plant regeneration.