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highest methane yield. It was also found that carbon to nitrogen (C/N) ratios for all mixtures fall within the optimal range (10:1 to 15:1) as compared to the control, which was out of range. This study was able to establish an optimal mixing ratio of the substrate and EM preferably to be 1:250 v/v at a dilution ratio of 1:4 m/m since it is observed to have the highest methane composition of 69% compared to the other treatments. The composted pyrethrum waste at all ratios can also be used as bio-fertilizer since the final COD of the compost is on an average of 134 g/L, suitable for soil conditioning.