Antioxidant Activity and Inhibitory Effect of Some Commonly used Medicinal Plants against Lipid Per-Oxidation in Mice Brain

  • A Khan
  • H Nazar
  • SM Sabir
  • M Irshad
  • SI Awan
  • R Abbas
  • M Akram
  • A Khaliq
  • JBT Rocha
  • SD Ahmad
  • F Malik
Keywords: Antioxidant activity, Balb c mice, iron chelation, phenolics, oxidative stress, medicinal plants.

Abstract

Background: The present study compares the protective properties of aqueous extracts of six medicinal plants, Phyllanthus emblica, Terminalia
chebula (black and yellow), Terminalia arjuna, Balsamodendron Mukul and Alium sativum against lipid per-oxidation in mice brain.
Methods: The antioxidant activities were analyzed by lipid per-oxidation assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay, total antioxidant
activity and metal chelation.
Results: The extracts (fruits and bark) showed inhibition against thiobarbituric acid reactive species (TBARS) induced by pro-oxidant (10 µM FeSO4) in mice brain. Moreover, the free radical scavenging activities of the extracts was evaluated by the scavenging of DPPH radical (IC50, 23.23±1.2 µg/ml (Phyllanthus emblica), 20.24±0.9 µg/ml (Terminalia chebula yellow) and 17.33±1.1 µg/ml (Terminalia chebula black), 19.44±0.45 µg/ml (Terminalia arjuna), 56.59±2.1 µg/ml (Balsamodendron Mukul) and . 200 µg/ml (Alium sativum).
Conclusion: The higher antioxidant and inhibitory effect of Terminalia chebula black in this study could be attributed to its significantly higher
phenolic content, Fe(II) chelating ability, reducing ability and free radical scavenging activity. Therefore oxidative stress in brain could be potentially
prevented by the intake of these plants.

Key words: Antioxidant activity, Balb c mice, iron chelation, phenolics, oxidative stress, medicinal plants.

Published
2014-10-27
Section
Articles

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eISSN: 0189-6016