Anti-inflammatory effect of Zanthoxylum bungeanum-cake-separated moxibustion on rheumatoid arthritis rats

  • Xulu Lei
  • Shirui Cheng
  • Honglian Peng
  • Qin He
  • Hongyu Zhu
  • Mengqian Xu
  • Qinqin Wang
  • Li Liu
  • Chengshun Zhang
  • Qizhi Zhou
  • Fang Zeng
  • Jian Ying
  • Haiyan Yin
  • Shuguang Yu
Keywords: Anti-inflammatory effect, Zanthoxylum bungeanum-cake-separated moxibustion, Aconitum carmichaeli-cake-separated moxibustion, Rheumatoid Arthritis.

Abstract

Background: Zanthoxylum bungeanum-cake-separated moxibustion (ZBCS-moxi), a kind of traditional therapy of moxibustion, has been used in China since 340 B.C. However, its mechanism remains unclear. So, this study was attempted to reveal the anti-inflammatory effect of ZBCS-moxi on rheumatoid arthritis (RA) rats.
Methods and Materials: Forty health SD female rats were randomly divided into 4 groups  (n=10/group): control group, model group, Zanthoxylum bungeanum-cake-separated moxibustion group (ZBCS-moxi group) and Aconitum carmichaeli-cake-separated moxibustion group (ACCS-moxi group). RA model was induced by injecting 0.1 ml Freund’s complete adjuvant (FCA) into the right hind paw of rats. Eleven days after CFA injection, the rats in ZBCS-moxi group received Zanthoxylum bungeanum- cake-separated moxibustion on Shenshu (BL23), Zusanli (ST36) acupoints and the dorsum right hind paw respectively for 3 weeks. The ACCS-moxi group was used as a positive control, while the rats were treated with Aconitum carmichaeli-cake-separated moxibustion on the same acupoints and courses. After a 3-week treatment, we investigated anti-inflammatory effect by measuring the paw volume,  observing the pathologic morphology of synovial membranes and detecting the concentration of IL-1β and TNF-α in serum.
Results: Compared to model group, the swollen paw volumes, the synovial hyperplasia and pannus formation of synovial membranes and the concentration of IL-1β and TNF-α in serum decreased obviously (P<0.01, P<0.05) in ZBCS-moxi group, as well as in ACCS-moxi group. However, the swollen paw volumes, the synovial hyperplasia and pannus formation of synovial membranes and the concentration of IL-1β and TNF-α in serum in ZBCS-moxi and ACCS-moxi group did not show significant differences (P>0.05)

Conclusions: ZBCS-moxi displays anti-inflammatory effect on RA rats via suppressing the expression of cytokines and has similar effect to ACCS-moxi.


Key words: Anti-inflammatory effect; Zanthoxylum bungeanum-cake-separated moxibustion; Aconitum carmichaeli-cake-separated moxibustion; Rheumatoid Arthritis.

Published
2016-02-18
Section
Articles

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eISSN: 0189-6016