In vitro antifungal activity and cytotoxicity screening of dry crude extracts from Brazilian Amazonia plants
Background: Antifungal multidrug resistance has been reported worldwide and has stimulated investigations of plant species for the treatment of candidiasis. In particular, in vitro antifungal activities and cytotoxicity of dry extracts from Ceasalpinia ferrea (tul.) Martius, Brosimum acutifolium Huber, and Salacia impressifolia (Miers) A.C. Smith were evaluated.
Materials and Methods: Minimum inhibitory concentrations (MIC) and minimum fungicide (MFC) values were established according to the protocol M27-A2 of the Clinical and Laboratory Standards Institute (CLSI). Subsequent evaluations were performed using strains of Candida albicans from the American Type Culture Collection (ATCC) 10231, clinical isolated Candida albicans, Candida glabrata (CCT) 0728, Candida krusei (FTI) CCT 1517, and Candida guilliermondii (CCT) 1890. Morphological changes were evaluated using scanning electron microscopy (SEM), and cytotoxicity was evaluated in murine L929 fibroblast cells after treatment with plant extracts.
Results: MIC values indicated antifungal potential of all three extracts against the main fungi that causes candidiasis.
Conclusion: In particular, C. ferrea showed promising antimicrobial potential against all strains. Hence, future studies are warranted to investigate pharmacologically active compounds from this extract that could be used as prototypes for drug development and/or as a source of raw pharmaceutical materials for the treatment of candidiasis.
Keywords: Candidiasis; Natural products; Toxicity
Copyright: Creative Commons Attribution CC.
This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.