African Journal of Traditional, Complementary and Alternative Medicines

Log in or Register to get access to full text downloads.

Remember me or Register

Isolation and characterization of antineoplastic alkaloids from Catharanthus roseus l. Don. cultivated in Egypt

K Shams, N Nazif, N Azim, K Shafeek, M Missiry, S Ismail, M Nasr


Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).

Key words: Catharanthus roseus L., Apocyanaceae, Vinblastine, Vincrisitine, Antileuckemic alkaloids, VLC, HPLC, HPTLC

AJOL African Journals Online