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African Journal of Traditional, Complementary and Alternative Medicines

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IN-VITRO EVALUATION OF ANTI-TRICHOMONAL ACTIVITIES OF EUGENIA UNIFLORA LEAF

Gabriel Femi Ibikunle, Adeleke Clement Adebajoa, Funmilayo Gladys Famuyiwa, Adetunji Joseph Aladesanmia, Clement Oladapo Adewunmib

Abstract


Eugenia uniflora, used ethnomedically in some tropical countries as an anti-infective, has shown anti-malarial and antitrypanocidal activities. Therefore using bioactivity guided fractionation, anti-trichomonal activity of E. uniflora leaf was investigated. Anti-trichomonal activities of leaf methanol extract and its fractions against Trichomonas gallinae as well as their cytotoxicities using an in vitro haemaglutination assay were determined. Anti-trichomonacidal activities of the extract improved on purification up to a stage. Subfractions E2-5 had LC50 and LC90 values of 4.77 - 5.28, 18.49 - 25.00 and 4.53 - 5.18, 18.32 - 19.07 ìg/ml at 24 and 48 hrs, respectively that were better than those of metronidazole. Further purification of E2-5 led to loss of activity suggesting that the active components were probably working synergistically and additively. Demonstration of low haemaglutination titre values of 0.00 – 5.33 by methanolic extract and its partition fractions suggested their low toxicity profile. The established safety of the leaf indicated that its anti-trichomonal activity was not due to non-specific cytotoxicity, hence could be used in ethnomedicine as an anti-trichomonal agent.

Keywords: Eugenia uniflora, leaf extract, Trichomonas gallinae, in vitro

Afr J Tradit Complement Altern Med. (2011) 8(2):170--176



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