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Activity guided isolation of chemical constituents from the biologically active methanol extract of Euphorbia schimperi C. Presl

S. Ahmed
M. N. e-Alam
R. A. Mothana
M. Yousaf
A. J. Al-Rehaily


In this study we investigated the chemical constituents of bioactive methanol extract of Euphorbia schimperi C. Presl. For this the methanol extract was fractionated into 20, 40, 60, 80% MeOH in CHCl3, and 100% MeOH fractions respectively by vacuum liquid chromatography. Excision wound surface of the animals were topically treated with these fractions at a dose of 100 mg/kg body weight for twenty days. Povidone-iodine ointment was used as a reference drug. Wound contraction measurement and period of epithelialization were used to assess the effect of fractions on wound repairing. The 100% MeOH fraction treated animals achieved significant (p < 0.001) value by showing 100% wound contraction and minimum period of epithelization (17.75±0.47) on the 20th day as compared to standard drug treated animals on the same day. The active 100% MeOH fraction was subjected to various chromatographic techniques led to the isolation of miquelianin (1), kaempferol 3-O-glucuronide (2) and quercitrin (3). Compounds (1-3) were isolated from this plant for the first time.


KEY WORDS: Euphorpia schimperi C. Presl, Wound healing, Chromatographic techniques, Chemical constituents


Bull. Chem. Soc. Ethiop. 2017, 31(3), 471-479.