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Bulletin of the Chemical Society of Ethiopia

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THE MASS OF CELLULAR RETINOIC ACID BINDING PROTEIN I INVESTIGATED BY 13C DEPLETION AND MASS SPECTROMETRY

Lin Zhang, Zeqing Song, Yanmei Wen

Abstract


The accuracy of mass spectrometry used to determine large molecular mass as proteins is often influenced by the isotopic compositions within a protein. Isotopic depletion is a powerful tool to resolve this problem. Using Fourier transform ion cyclotron resonance mass spectrometer, we investigated the 13C depleted cellular retinoic acid binding protein I, the measured mass accuracy is increased compared with the natural protein
KEY WORDS: Molecular mass, Mass accuracy, Isotopic depletion, Monoisotopic


Bull. Chem. Soc. Ethiop. 2009, 23(1), 101-103.



http://dx.doi.org/10.4314/bcse.v23i1.21304
AJOL African Journals Online