Role of zontivity loaded by PLGA coated by TPGS in ameliorating induced cardiovascular and pulmonary disorder in adult male

Abstract

This study was aimed to evaluate the role of PLGA nanoparticle that was prepared by two procedures involved nanoprecipitation methods. The study evaluated the effect of the organic phase with different concentration and different type of aqueous phase on particle size and Encapsulation Efficiency (EE), Polydispersity index (PDI) and Loading Efficiency (ID). Our data showed that using of acetone alone as well as when mixed with Dimethyl Sulfoxide (DMSO) and 0.03% TPGS that produced small diameter particle size as well as with suitable ID and EE reached ED to 110 nm, 9.89 %, and 95.9% , respectively.
Fifty adult male rats were divided randomly in to five equal groups (5 animal /each group), the first group (CNTR) was a negative control , the second group (T1) administrated high fat die 2 % with 0.5 % of H2O2 as a positive group, third group (T2) was received high fat die 2 % with 0.5 % of H₂O₂ treated with a daily dose of 1.1mg/kg empty nanoparticles, fourth group (T3) administrated high fat die 2 % with 0.5 % of H₂O₂ and treated with a daily dose of 0.38mg/kg of ordinary Vorapaxar, and five group administrated high fat die 2 % with 0.5 % of H₂O₂ and treated with a daily dose of Zontivity Loaded by PLGA at dose of 0.11mg/kg. The experiment was persistent for 10 weeks. In vivo study showed that Zontivity loaded PLGA appear has ability to promote antioxidant parameter as SOD and GPX, as well as reduction serum troponin. The data of serum chloride showed a significant reduction in positive control and empty PLGA groups than treated groups.
Moreover, histopathological changes of nanoparticles presented a cardiac hypertrophy and infiltration of inflammatory cells that didn’t show any vaculation or necrosis in comparison with other groups, and that may support the histopathological architecture of pulmonary tissue which showed only minor thickening in alveolar septa.