HIV infection and immunosenescence: A comparison of immune status and t cell homing markers between HIV infected and uninfected elderly individuals in Kisii, Kenya
Objectives: To examine if differences exist among people aged ≥50 years in Tcell phenotypes frequencies and homing capacity between human
immunodeficiency virus (HIV) infected and HIV uninfected controls that impacts Immunosenescence.
Design: Laboratory-based cross-sectional study.
Setting: Immunology laboratory at Kisii Teaching and Referral Hospital (KTRH) in Kisii, Kenya.
Subjects: Asymptomatic elderly HIV infected patients attending HIV clinic and HIV uninfected controls all aged 50 years. Age used is according to the criteria of the American Centres for Disease Control and Prevention (CDC) which describes HIV/AIDS infected individuals aged 50 years as “elderly”.
Procedure: T-cell subset counts were resolved utilising FACScan flow cytometer and outcome processed employing FlowJo computer programme.
Differences in cell count and percentages of T lymphocytes were analysed utilising Student’s t-test and linear regression based on HIV status and age Main outcome measures: Cell count and percentage of T lymphocytes, CD4/CD8 ratio and age
Results: Significant difference were found in CD45+ (P = 0.045 and 0. 003), CD3+ (P=0.001), CD4+ (p<0.001), CD8+ (p<0.001), CD8+CCR7- (p<0.001) and CD4+CCR7- (p<0.001 and P=0.002) cells between HIV infected and uninfected people.
Conclusion: T-cell phenotypes frequencies and homing capacity are significantly altered among elderly HIV infected compared to the HIV uninfected controls leading to greater impairment of the T cell apportionment among older HIV infected and consequently HIV accelerates