Cysteine peroxidase activity in rat blood plasma

  • AV Razygraev
Keywords: aminothiol, stoichiometry, 5, 5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent

Abstract

Early studies recognized that the human extracellular glutathione peroxidase may function cysteine peroxidase (cysteine:H2O2 oxidoreductase). The aim of the present study was the hypothesis of cysteine:H2O2 oxidoreductase activity in rat plasma. The rat plasma found to be able to accelerate greatly the H2O2-dependent oxidation of cysteine. The activity was a characteristic of a protein fraction precipitated at 30—44% ammonium sulfate saturation, and the specific activity in protein fraction was significantly higher than in plasma. Cysteine:H2O2 oxidoreductase reaction stoichiometry (2:1 mole ratio) was detected for the catalyzed interaction between cysteine and H2O2. Estimation of cysteine peroxidase activity may be useful in research on the functioning of the antioxidant system.

Keywords: aminothiol, stoichiometry; 5,5'-dithiobis(2-nitrobenzoic acid); Ellman's reagent

Published
2013-10-25
Section
Articles

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eISSN: 1110-6859