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Background: Type 2 diabetes mellitus is a multifactorial and heterogenic disease with a complex etiology. In recent decades the association of a large number of genes has been shown with T2DM. CAPN10 gene was the first T2DM candidate gene identified through genome-wide screening and positional cloning, and among all identified genes until now, TCF7L2 gene has shown most association with T2DM. The aim of this study was to investigate the association between TCF7L2 rs11196205(C/G) and CAPN10 rs3792267 (G/A) with T2DM in a subset of Iranian population from Khuzestan province. It should be noted that this is the first report of TCF7L2 polymorphism rs11196205with T2DM in Iran.
Subjects and methods: A case-control association study was performed using 150 T2DM patients and 150 controls. Genotyping for TCF7L2 rs11196205 was done by Tetra-Primer ARMS-PCR and for CAPN10 rs3792267 was done by PCR-RFLP Technique.
Results: Statistical analyses were carried out using SPSS version 16. In examining TCF7L2 rs11196205 based on the genotype GG, results for CG genotype were, 95%CI = (0.5–1.7), OR = 0.92, P-value = 0.79 and for genotype CC were, 95%CI = (0.94–3.92), OR = 1.92, P-value = 0.07. in examining CAPN10 rs3792267 based on the genotype AA, results for GG genotype were, 95%CI = (0.55–6.8), OR = 1.93, P-value = 0.31 and for genotype GA were, 95%CI = (0.43–5.64), OR = 1.55, P-value = 0.5. So, in both polymorphisms, none of the alleles or genotypes had significant statistical differences between case and control groups (P > 0.05).
Conclusion: Our results showed that TCF7L2 rs11196205 and CAPN10 rs3792267 (SNP- 43) polymorphisms are not associated with the risk of T2DM in the studied population.
Keywords: Association study, CAPN10, TCF7L2, Type 2 diabetes mellitus, rs11196205, rs3792267