PROMOTING ACCESS TO AFRICAN RESEARCH

Global Journal of Medical Sciences

Log in or Register to get access to full text downloads.

Remember me or Register



In vitro effects of aspirin and paracetamol on human erythrocyte glutathione-s-transferase activity

A. A. Uwakwe, M. O. Monanu

Abstract


The activity of glutathione-S-transferase of human erythrocytes was monitored spectrophotometically in the presence and absence of two analgesics (aspirin and paracetamol). Five different concentrations (1.0, 3.0, 5.0, 7.0, 9.0 and 10.0 mg/ml) of each analgesic were used which covers the reported therapeutic and toxic concentration range of the drugs. The drugs were observed to significantly (P < 0.05) inhibit the enzyme. Inhibitions greater than 42.0% were observed at 10mg/ml concentrations of the two drugs. Enzyme inhibition studies demonstrated a competitive inhibition pattern for both aspirin and paracetamol. The inhibition pattern was interpreted as indicative of a higher degree of inhibition at reduced levels of the enzyme's substrate, glutathione (GSH).


(Global Journal of Medical Science, 2004, 3 (1&2): 33-36)



http://dx.doi.org/10.4314/gjms.v3i1.10139
AJOL African Journals Online