High performance liquid chromatographic (HPLC) separation of some fatty acids as naphthyl methly derivatives using resin supported 1-naphthalene methanol as the derivatization reagent

Abstract

Cation exchange resin in the sulphonyl chloride form was prepared by reacting cation exchangers in the sodium form with calculated amount of phosphorus pentachloride by refluxing. The sulphonyl function on the resin serves as an anchor through which naphthalene methanol moiety was bonded to the imobile resin backbone. The resin supported 1-naphthalene methanol was subsequently employed to derivatize some fatty acids. The reagent imparted fluorescence property on the final derivatives. These derivatives were obtained in pure form and simply isolated by filtration. The derivatives absorbed UV-radiation strongly at 254nm and direct HPLC analysis was found possible without interference from excess reagent; This indicated that the resin supported 1-naphthalene methanol may be employed for heterogeneous on-line pre-or post column derivatization of fatty acids. To whom correspondence should be addressed.

Keywords: Resin, Moiety, Derivative, Fluorescence, Heterogeneous.


(Global Journal of Pure and Applied Sciences: 2003 9(2): 239-248)