Current Stereological Methods and Tools for Simple Quantification of Biological Structure: A short Review

  • A N Makanya
  • C N Warui
  • L M Karlsson


A statement like “the cells were more numerous” conveys little meaning unless numbers are attached to the observations. Fortunately, a set of general methods, known as stereology, may be used to quantify tissue structural changes due to growth, development or disease. Parameters like volume, surface area, length or number, of target structure are estimated using sampling probe has random position and whenever appropriate, random orientation, recent design-based stereology enjoys the advantage of being unbiased that is without systematic error. The structure under study doesn't have to be random: the required element of randomness is provided by the design sampling protocol. Stereology has, however rarely been used in the developing world. The purpose of this presentation is to give a brief review of recent stereological methods for unbiased quantification of biological structures. A prerequisite step undertaken is procurement of the required number of specimens or blocks that are convenient for the investigation. In particular stereological methods give definite and precise descriptions of the changes in biological tissue. Contemporary researchers in the developing world are encouraged to employ stereological techniques in their research.

The Kenya Veterinarian Vol. 27 2004: pp. 113-117

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eISSN: 0256-5161