Study on the Effects of Dictyota ciliolata Polysaccharides on Proliferation and AntiOxidation Status of Cyprinus carpio (common carp) leukocytes

Abstract

The brown seaweed (Dictyota ciliolataa Sonder ex Kutzing) has been identified as a valuable source of anionic polysaccharides with  remarkable biological properties that may render them suitable for application in aquaculture. In this study, the chemical composition of  D. ciliolata polysaccharides (DCP) was analysed using spectrophotometry and HighPerformance Anion-Exchange Chromatography/  Pulsed Amperometric Detection (HPAEC-PAD). The result showed that DCP had a total carbohydrate content of 56.56 ± 1.17 %, sulphate  content of 4.58 ± 0.26 %, uronic acid content of 6.54 ± 0.26 %, and protein content of 7.31 ± 0.24 %. HPAEC-PAD revealed that DCP  composed of fucose (27.16 ± 4.27 mg/l), galactose (11.47 ± 1.38 mg/l), arabinose (11.88 ± 1.24 mg/l) and glucose (1.64 ± 0.07 mg/l). DCP  was non-toxic to carp leukocytes at dosages ≤ 50 µg/ml but was found to induce cell proliferative activity. Cellular antioxidant assays  showed that DCP did not disrupt glutathione and superoxide dismutase activities in carp leukocytes cells when compared to the control. However, a decrease in malondialdehyde levels in carp leukocytes cells indicated that DCP did not induce oxidative stress. Therefore, DCP  is a promising antioxidant source that could be effectively utilised in enhancing the health status of cultured fish species.