Nigerian Journal of Clinical Practice

Log in or Register to get access to full text downloads.

Remember me or Register

Effects of Ozone and Photo‑Activated Disinfection against Enterococcus faecalis Biofilms In Vitro

Ö Tuncay, AN Dinçer, A Kuştarcı, Ö Er, G Dinç, S Demirbuga


Objectives: The purpose of this study was to compare the antibacterial effects of gaseous ozone (O3) and photo‑activated disinfection (PAD) methods against Enterococcus faecalis (E. faecalis) biofilms.

Materials and Methods: Sixty‑five human mandibular premolars with straight root canals were selected. After root canal preparation, the samples were sterilized and placed into eppendorf tubes with 1 mL brain heart infusion broth containing 1.5 × 108 colony‑forming units (CFUs)/mL of E. faecalis. The contaminated samples were then divided into four groups (n = 15) according to the disinfection method used: Group 1, Saline (positive control); Group 2, NaOCl (negative control); Group 3, Gaseous O3; and Group 4, PAD. Three non‑contaminated teeth were used to control the infection and sterilization process. The CFUs were counted and the data were analyzed statistically.

Results: There was a statistically significant difference between the experimental and control groups (P < 0.05). The saline group had the highest number of remaining microorganisms. Complete sterilization was achieved in the 2.5% NaOCl group. There were no statistically differences between PAD and gaseous O3 (P > 0.05).

Conclusion: Both PAD and gaseous O3 have a significant antibacterial effect on infected root canals. However, 2.5% NaOCl was superior in terms of its antimicrobial abilities compared with the other disinfection procedures.

Keywords: Biofilm, Ozone, Photo‑activated Disinfection, Root Canal Disinfection
AJOL African Journals Online