Oral mucosa and lung cancer: Are genetic changes in the oral epithelium associated with lung cancer?

  • N Kömerik
  • E Yüce
  • N.S. Calapoğlu
  • P.A. Koşar
  • M Çakir
  • G Koparan
Keywords: Lung cancer, murine double minute 2, oral epithelium, p53, polymorphism

Abstract

Aim: To compare genetic aberrations in the oral epithelium of lung cancer patients with those without cancer.

Subjects and Methods: Buccal smears were performed to collect oral epithelium from each of the participants (smoker cancer patients n = 50, smoker control subjects n = 40, and nonsmoker control subjects n = 25). Cytogenetic changes in the samples were detected by micronuclei assay, whereas p53 and murine double minute 2 (MDM2) polymorphisms were genotyped using polymerase chain reaction‑restriction fragment length polymorphism.

Results: p53 codon 72 polymorphism was seen in 44% of cancer patients versus 12.5% in smokers and 12% in nonsmokers of the control group. Similarly, MDM2 single nucleotide polymorphism 309 polymorphism was seen in 34% of patients with lung cancer as opposed to 12.5% of smokers (P = 0.038) and 8% of nonsmokers (P = 0.019) of the control group.

Conclusion: A higher proportion of individuals with lung cancer demonstrate genetic damage to oral mucosa compared to those without cancer.

Keywords: Lung cancer, murine double minute 2, oral epithelium, p53, polymorphism

Published
2016-12-13
Section
Articles

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eISSN: 1119-3077