Intralipid and Caffeic Acid Phenethyl Ester Reverse the Neurotoxic Effects of Organophosphate Poisoning in Rats

Abstract

Background: Organophosphate (Op)-containing herbicides continue to be widely used in the world. Although its usage and intoxication  are widespread, the studies on organophosphate‑induced neurotoxicity and treatment protocols are very few in the literature.

Aims: This  study aimed to investigate any potential effects of caffeic acid phenyl ester with/without intralipid on neurotoxicity produced by acute intoxication of glyphosate isopropylamine in an experimental rat model.

Materials And Methods: Forty‑nine wistar albino rats  were randomly allotted into seven experimental groups: I, control; II, intralipid (IL); III, caffeic acid phenyl esther (CAPE); IV, glyphosate  isopropylamine (GI); V, GI + IL; VI, GI + CAPE; and VII, GI + IL + CAPE. Total antioxidant and oxidant status levels were gauged, and the  oxidative stress index was calculated in the serum samples. On the other hand, the tissues were analyzed with hematoxylin‑eosin (HE) staining protocol and counted up by immunohistochemical method. Statistical evaluations were conducted using SPSS 11.5 for Windows  (SPSS, Chicago, IL, USA).

Results: Compared to the control, IL, and GI + IL + CAPE groups, the GI group significantly decreased the total  antioxidant levels in brain tissues. In a supportive nature, a significant increase in the oxidative site index (OSI) in the GI group compared  to other groups. Especially standing out point of these findings is the significant difference between the GI + IL + CAPE and the GI group.  Parallelly, histopathological analysis extended severe neurotoxicity in the GI group. Neurotoxic status was reduced significantly in the GI  + CAPE + IL group. The histopathologic examinations confirmed biochemical results. The results also revealed that CAPE and IL, probably  their antioxidant effects, have a rehabilitative effect on neurotoxicity caused by GI.

Conclusion: Therefore, CAPE and IL may function as  potential cleansing and scavenger agents for supportive therapy regarding tissue damage or facilitate the therapeutic effects of the  routine treatment of the patient with GI poisoning.