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In vitro Heparin Precipitation in the Plasma of Euthyroid women with Recurrent Miscarriage and Unexplained Infertility
OBJECTIVE: Women with elevated Lp(a), who are susceptible to atherosclerosis, get to reduce, their cardiovascular disease by in-vivo administration of low dose heparin. And history of recurrent miscarriage associated with auto antibodies have had a high rate of life births in subsequent pregnancies when they were treated with low dose aspirin together with low dose heparin. An in-vitro assay thus provide basis for the intricate interaction.
DESIGN: Two hundred and ten (210) healthy volunteered Euthyroid women were used for this study. They were aged between 21-35 years, 90 Women served as test groups and 120 women served as control. Antimicrosomal autoantibody (Anti-TPO) ELISA assay was determined. The plasma treatment at the different PH with heparin and aspirin in physiological buffers were carried out.
RESULT: At PH5.0 (i.e. the Uterus physiological PH) using 100i.u/ml heparin in 0.2M acetate buffer yielded 70% precipitate compared to treatment with 250i.u/ml heparin in 0.2M buffer that yielded 40% precipitate while 100-i.u/ml heparin with 80mg aspirin treatment yielded 65% precipitate in the test group samples that were positive to Anti-TPO. Whereas in the 0.2ml acetate buffer alone (without heparin and aspirin) no precipitate was formed, compared to the precipitate formed with plasma of the control women yielding 15% using 100i.u/ml heparin ,5% with 250i.u/ml heparin and 12% 100-i.u/ml heparin with 80mg aspirin treatment respectively.
At PH 4.6 (i.e blood physiological PH ) for the various treatment showed there were increases in percentage precipitate, more in the subjects than in the control groups. In the unexplained and spontaneous abortion, groups with the treatment of 100iu/ml Heparin in 0.2M acetate buffer gave highest precipitate compared to other treatment in in-vitro study.
CONCLUSION: This study has further supported that Heparin in addition to its anticoagulant action, may act to reduce fetal loss by binding to antimicrosomal, (anti-phospholipids) antibodies, thereby protecting the trophoblast phospholipids attack, and thereby promote successful implantation in early pregnancy.
Keywords: Heparin, Immunogens, antimicrosomal antibodies, Infertility
Nigerian Journal of Health and Biomedical Science Vol. 6 (1) 2007: pp. 1-4