Nigerian Journal of Natural Products and Medicine

The AJOL site is currently undergoing a major upgrade, and there will temporarily be some restrictions to the available functionality.
-- Users will not be able to register or log in during this period.
-- Full text (PDF) downloads of Open Access journal articles will be available as always.
-- Full text (PDF) downloads of subscription based journal articles will NOT be available
We apologise for any inconvenience caused. Please check back soon, as we will revert to usual policy as soon as possible.

DOWNLOAD FULL TEXT Open Access  DOWNLOAD FULL TEXT Subscription or Fee Access

Quality control of Harpagophytum procumbens products using High Pressure Liquid Chromatography-Diode Array Detection (HPLC-DAD)

S Chigome, MN Nindi, NM Munkombwe


Iridoid and phenylethanoid glycosides were used as compounds to monitor the quality of Devil’s Claw (Harpagophytum procumbens) based products in this investigation. A HPLC-DAD method was developed for the determination of the phenylethanoid glycoside isoacteoside and the iridoid glycoside harpagoside in commercially available Devil’s Claw products. The limits of detection (LOD) obtained were 1.08 μg/ml for harpagoside and 1.70 μg/ml for
isoacteoside. Limits of quantification (LOQ) of harpagoside and isoacteoside were 3.59 μg/ml and 5.65 μg/ml respectively. The harpagoside and isoacteoside contents in the ethanol extracts of the commercial products were found to be in the range of 2.2 % - 3.9 % and 0.22 % - 0.50 % respectively. 

Keywords: Harpagophytum procumbens, phenylethanoid glycosides, iridoid glycosides, harpagoside, HPLC-DAD profiles

Full Text:

No subscription journal articles available during site upgrade.
AJOL African Journals Online