PROMOTING ACCESS TO AFRICAN RESEARCH

Nigerian Quarterly Journal of Hospital Medicine

Log in or Register to get access to full text downloads.

Remember me or Register



DOWNLOAD FULL TEXT Open Access  DOWNLOAD FULL TEXT Subscription or Fee Access

Mucuna pruriens protects the testes from Quinine-induced testicular toxicity in Sprague-Dawley rats

O.J. Olabanji, E Akang, O.E. Yama, T.O. Kusemiju, A.A. Oremosu, A.A. Osinubi

Abstract


Background: Quinine (QU) has been identified as a testicular toxicant that leads to infertility. However some plant extracts have demonstrated protective effects on the testes.

Objective: To determine the effect of Mucuna pruriens (MP) seed extract on the testes and its protective role on QU-induced testicular damage.

Methods: Twenty male rats weighing between 120-150 g were used for the study. They were divided into four groups of five rats each. Group I (control) administered distilled water for 8 weeks. Group II received 10 mg/kg of QU only for 8 weeks. Group III rats were treated with QU and MP seed extract at 10 and 75 mg/kg concurrently for 8 weeks. Group IV received 75 mg/kg of MP seed extract only for 8 weeks. At the end of the experimental durations animals were sacrificed by cervical dislocation. Testes harvested, processed for testicular: glutathione (GSH), catalase activity (CAT), superoxide dismutase (SOD) activity, testicular malondialdehyde (MDA) and microscopic studies. Blood samples collected for testosterone assay.

Results: There were significant increases in serum testosterone and testicular antioxidants (GSH, CAT and SOD) levels and a decrease in MDA levels in rats treated with MP only and parallel doses of QU and MP for 8 weeks when compared to control and animals treated with QU alone. The histology showed corresponding improvement compared to control and QU treated rats.

Conclusion: MP increases testosterone levels and testicular antioxidant enzymes as well as protecting the testis against QU induced testicular damage.

Keywords: Mucuna pruriens, Testis, Quinine, Infertility




AJOL African Journals Online