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The determination of carnitine in body matrices has proved a difficult task over the years because of the errors inherent in the various radiometric methods that have been used. In this research the potential sources of error in the routinely used radio-isotopic assays for carnitine have been recognised and eliminated by the carefuI as"f'SSlN'llt of each step in the process. The assay described in this paper allows for quick. reproducible and reliable determinations of free and esterified carnitine in two body matrices, viz. sermn and urine.