The Effects of Leukocyte- and Platelet-Rich Plasma (L-Prp) and Pure Platelet-Rich Plasma (P-Prp) in a Rat Endometriosis Model

  • Ali Doğukan Anğın
  • İsmet Gün
  • Yasemin Alan
  • Önder Sakin
  • Muzaffer Seyhan Çıkman
  • Zehra Meltem Pirioğlu
  • Kayhan Başak
  • Ahmet Kale
  • Pınar Kaygın
  • Serpil Oğuztüzün
  • Murat Alan
Keywords: L‑Prp; P‑Prp; VEGF; endometriosis.


Objective: We aimed to investigate the effect of platelet‑rich plasma (PRP) derivatives, which can be produced from the patient’s blood and have minimal side effects, on endometriosis.
Methods: To our knowledge, this is the first study in the literature that studies the relationship between PRP and endometriosis. Endometriosis foci were created in the first operation. In the second operation (30th day), four groups were formed wherein group 1 (n = 8) was administered saline, group 2 (n = 7) leukocyte and platelet‑rich plasma (L‑PRP), group 3 (n = 8) pure platelet‑rich plasma (P‑PRP) and group 4 (n = 10) was used to obtain PRP. In the last operation (60th day), the endometriotic foci was measured and then excised.
Findings: There was no statistically significant difference between the pre and post volumes of the endometriotic foci, between their volume differences, and volume difference rates (P > 0.05). However, it was observed that existing implant volumes in all groups decreased statistically significantly within their groups by the end of the experiment compared to the previous volumes (P < 0.05).
Conclusion: When the implants were assessed through histopathological scoring in terms of edema, vascular congestion, inflammatory cell  infiltration, hemorrhage, epithelial line, and hemosiderin accumulation, and immunohistochemical staining was assessed in terms of VEGF, there was no significant difference in the comparison between the groups. Although L‑PRP and P‑PRP generated more reduction in the endometriosis foci, they did not create any statistical differences.

Key words: L‑Prp; P‑Prp; VEGF; endometriosis.


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eISSN: 0189-5117