In vitro Antitumor Activities of Platycarya strobilacea Sieb et Zucc Infructescence Extracts
Purpose: To evaluate the antitumor activities of Platycarya strobilacea infructescence extracts in A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB cell lines.
Methods: The total amount of phenolics in P. strobilacea infructescence based on three solvent extracts (methanol, ethyl acetate and water) was measured using Folin-Ciocalteu reagent method. The cytotoxic effect of the various solvent extracts and tannins on five cancerous cell lines (A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB) were assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide [MTT].
Results: Ethyl acetate extract had the highest phenolic content (725.02 mg GAE/g), followed by methanol extract (627.64 mg GAE/g). Among the extracts tested, ethyl acetate extract had effective anticancer activity against A549, HepG2, HCT116 and U2OS-NKFB cells with IC50 values of 40.1, 42.6, 40.1 and 73.4 μg/mL, respectively. Methanol extract had the strongest cytotoxic effect on SH-SY-5Y cell with IC50 value of 31.2 μg/mL. The number of surviving cancerous cells decreased as the concentration of the ethyl acetate and methanol extracts increased.
Conclusion: The study confirms the antitumor activities of ethyl acetate and methanol extracts of P. strobilacea infructescence. Thus, the plant extracts constitute potential therapeutic materials that require further studies and development.
Keywords: Platycarya strobilacea, Antitumor, Phenolic compounds, Tannins, MTT assay
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