Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register

Simultaneous Determination of Ciprofloxacin and Tinidazole in Tablet Dosage Form by Reverse Phase High Performance Liquid Chromatography

T Sirisha, BM Gurupadayya, S Sridhar


Purpose: To develop a simple, sensitive and specific liquid chromatographic method with PDA detection for the simultaneous estimation of ciprofloxacin and tinidazole in tablet dosage form.
Methods: Separation was achieved with an Agilent XDB C18, 250 × 4.60 mm 5 μ column, low pressure gradient mode with a ambient temperature and mobile phase comprising acetonitrile water containing 0.1 % orthophosphoric (20:80). The flow rate was 1 ml/min and eluent was monitored spectrophotometrically at 316 nm.
Results: The selected chromatographic conditions effectively separated ciprofloxacin and tinidazole with retention time of 3.036 and 4.224 min, respectively. Linearity for ciprofloxacin and tinidazole was in the range 50 - 100 and 60 - 120 μg/ml, respectively. Regression coefficient was 0.999 for both ciprofloxain and tinidazole while recovery waas 100.19 - 100.92 and 99.36 - 100.48 % for ciprofloxacinand tinidazole, respectively. Relative standard deviation (RSD) of intra- and inter-day precision was < 2
% for both drugs.
Conclusion: The developed method is precise, accurate, reproducible and specific and it can also be used for routine simultaneous quality-control analysis of ciprofloxacin and tinidazole in combination tablets.

Keywords: High performance liquid chromatography, Ciprofloxacin, Tinidazole, Simultaneous determination.
AJOL African Journals Online