Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register

Influence of Extraction Parameters on Hydroalcohol Extracts of the Stem Bark of Rapanea ferruginea Mez Using Myrsinoic Acid B as Marker

T Baccarin, RA Ferreira, VF Gazoni, RA Yunes, A Malheiros, RM Lucinda-Silva


Purpose: To evaluate the influence of extraction parameters on standardization of hydroalcoholic extract of Rapanea ferruginea Mez.
Methods: A 33 factorial design was used to evaluate the influence of alcohol concentration (50, 70 and 90 % v/v), extraction time (2, 6 and 10 h), and particle size of the herbal drug (0.25, 0.5 and 1.0 mm) on the pH, dry residue and myrsinoic acid B (MAB) content of hydroalcoholic extracts by high performance liquid chromatography (HPLC) method.
Results: For the extracts, there was inverse correlation between dry residue and MAB content (p < 0.8046). The parameter that had the greatest influence on extraction process was alcohol concentration (p < 0.007) followed by particle size (p < 0.046). However, extraction time did not influence the process in terms of MAB (p < 0.675). Although, the highest MAB content was obtained with 70 % v/v alcohol and extraction time of 6 h, the best methodology for extractive preparation was the use of 90 % v/v alcohol and extraction time of 2 h because it resulted in a higher yield of MAB per solution volume, i.e,, with regard to MAB content and mass yield.
Conclusion: The results indicate that 90 % v/v alcohol and extraction time of 2 h have optimum influence on extraction process and were the best conditions for obtaining a standardized extract of R. ferruginea, using the method employed in this study. Thus, the findings of this study are a contribution to the development of a new phytopharmaceutical intermediate product.

Keywords: Rapanea ferruginea, Hydroalcoholic extract, Myrsinoic acid B, Phytopharmaceutical, Factorial desing, Standardization
AJOL African Journals Online