Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register

Extract of Zanthoxylum bungeanum maxim seed oil reduces hyperlipidemia in hamsters fed high-fat diet via activation of peroxisome proliferator-activated receptor γ

G Chen, X Gao, KS Zhen, ZY Yin, XX Zheng


Purpose: To investigate the anti-hyperlipidaemic effect of extract of Zanthoxylum bungeanum Maxim. seed oil (EZSO) on high-fat diet (HFD)-induced hyperlipidemic hamsters.
Methods: Following feeding with HFD for 30 days, hyperlipidemic hamsters were intragastrically treated with EZSO for 60 days. Serum levels of triglyceride (TG), total cholesterol (TC), low-density-lipoproteincholesterol (LDL-C), nitric oxide (NO) and malondialdehyde (MDA) were analyzed. Protein expression and location of peroxisome proliferator-activated receptor γ (PPARγ) in liver were determined by Western blot and immunohistochemical assays, respectively.
Results: EZSO at 5 and 10 g/kg significantly reduced levels of TG by 26 and 23 % (p < 0.05), TC by 19 % (p < 0.01) and 13 % (p < 0.01), LDL-C by 18 % (p < 0.05) and 21 % (p < 0.01), NO by 15 % (p < 0.01) and 31 % (p < 0.01), and MDA by 16 % (p < 0.05) and 30 % (p < 0.01), respectively, in serum of hyperlipidemic hamsters. However, EZSO did not show significant effect on HDL-C level in serum. Furthermore, EZSO at 5 and 10 g/kg markedly promoted protein expression of PPARγ by 71 % (p < 0.05) and 102 % (p < 0.01) in liver tissue of hyperlipidemic hamsters. EZSO also notably increased the content of PPARγ protein in the nucleus of liver cells of hyperlipidemic hamsters.
Conclusion: These findings suggest that EZSO can reduce hyperlipidemia and improve oxidative stress in hyperlipidemic hamsters through activation of PPARγ, and that EZSO is a promising novel hypolipidemic health product.

Keywords: Zanthoxylum bungeanum, Peroxisome proliferator activated receptor γ, Hyperlipidemia, Hamster, High-fat diet
AJOL African Journals Online