Neuroprotective Effect of Sargassum thunbergii (Mertens ex Roth) Kuntze in Activated Murine Microglial Cells
Purpose: To evaluate the anti-oxidant and anti-neuroinflammatory effects of the Sargassum thunbergii extract (Mertens ex Roth) Kuntze (STE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells in vitro.
Methods: STE antioxidative activity was evaluated with an Electron Spin Resonance (ESR)
spectrometer, which measured 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. Cell viabilities were estimated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assays. LPS-stimulated BV-2 microglia were used to study the expression and production of inflammatory mediators, such as nitric oxide (NO), inducible NO synthase (iNOS) and tumor necrosis alpha (TNF-α).
Results: LPS treatment, following STE pretreatment, decreased NO production by 13 ~ 65% in a dosedependent manner (p < 0.001 at 20, 40, 80 and 100 μg/mL), and was associated with the downregulation of inducible nitric oxide synthase (iNOS) expression. STE also attenuated the TNF-α soluble protein by 16 ~ 47% (p < 0.01at 20, 40 and 80 μg/mL) in activated murine microglia. Furthermore, the DPPH-generated free radicals were inhibited by STE concentration-dependently.
Conclusion: STE has therapeutic potential in the prevention or treatment of neurodegenerative and oxidative stress-related disorders.
Keywords: Sargassum thunbergii, Neurodegenerative diseases, Anti-inflammatory, Microglial cells, Inducible nitric oxide synthase (iNOS), Tumor necrosis factor (TNF)-α
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.