Enhanced Production of Palmarumycins C12 and C13 in Mycelial Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 with In situ Macroporous Resin Adsorption
Abstract
Purpose: To evaluate in situ macroporous resin adsorption for enhancement of palmarumycins C12 and C13 production in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12.
Methods: Ten macroporous adsorption resins (D-101, D1300, HPD-100, X-5, AB-8, DM130, ADS-17, DA-201, NKA-9 and S-8) were tested for absorption of palmarumycins C12 and C13 in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12.
Results: Among the resins, DA-201 showed the most significant enhancing effect on accumulation of palmarumycins C12 and C13 in mycelial liquid culture of endophytic fungus Berkleasmium sp. Dzf12. When resin DA-201 was applied to the medium at a concentration of 4.17 % on day 11 and then harvested on day 15, the maximal yield of palmarumycins C12 and C13 was 149.86 and 55.78 mg/L, which correspond to 70.69- and 1.82-fold higher than for control (2.12 and 30.70 mg/L, respectively). Approximately 95.81 % of palmarumycin C12 and 87.20 % of palmarumycin C13 were distributed in resin DA-201.
Conclusion: The results indicate that in situ resin adsorption is an effective strategy for enhancing the production of palmarumycins C12 and C13, and also for facilitating their recovery from the mycelial liquid culture of Berkleasmium sp. Dzf12.
Keywords: Endophytic fungus, Berkleasmium sp. Dzf12, Spirobisnaphthalene, Palmarumycin, Macroporous adsorption resins, Mycelial liquid culture
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