Chemical Composition and Antifungal Properties of Essential Oil of Origanum vulgare Linnaeus (Lamiaceae) against Sporothrix schenckii and Sporothrix brasiliensis

  • CSF Couto
  • NRB Raposo
  • S Rozental
  • LP Borba-Santos
  • LML Bezerra
  • PA de Almeida
  • MAF Brandão
Keywords: Antifungal Activity, Essential Oil, Gas Chromatography, Origanum vulgare, Sporotrichosis

Abstract

Purpose: To evaluate the effect of the essential oil of Origanum vulgare Linnaeus (Lamiaceae) on the growth of Sporothrix schenckii and Sporothrix brasiliensis.

Methods: The chemical composition of the essential oil was investigated by gas chromatography/flame ionization detector (GC-FID). The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were determined by broth micro-dilution method. Scanning electron microscopy (SEM) was also performed to reveal morphological alterations in Sporothrix spp. cells.

Results: The major components of the essential oil were γ-terpinene (30.5%), carvacrol (15.7 %) and 4-terpineol (13.0 %). γ-Terpinene showed potential antifungal activity with MIC ranging from 62.5 to 500.0 μg mL-1 for S. schenckii, and 125.0 to 250.0 μg mL-1 for S. brasiliensis. SEM micrographs revealed morphological alterations in hyphae and reduction of the adhered conidia numbers.

Conclusion: Origanum vulgare Linnaeus essential oil possesses potential antifungal activity, and can, therefore, can be developed as an alternative agent for the treatment of sporotrichosis.

Keywords: Antifungal Activity, Essential Oil, Gas Chromatography, Origanum vulgare, Sporotrichosis

Published
2015-08-06
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996