Tropical Journal of Pharmaceutical Research

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Effect of Chronic Alcohol Consumption on Phosphatidylcholine Hydroperoxide Content of Rat Liver and Brain

CW Pyun, PK Mandal, GE Hong, CH Lee


Purpose: To investigate the correlation between alcohol-induced oxidative stress and tissue phosphatidylcholine hydroperoxide (PC-OOH) content of rat liver and brain.

Methods: Ten Wistar rats were divided into two groups: one group was given 20 % ethanol (5 g/kg) and the other the same volume of normal saline, orally once a day for 6 weeks. Catalase activity, malondialdehyde (MDA) content, total antioxidant capacity (TAC) and PC-OOH content of liver and brain were determined.

Results: The ethanol-treated group had lower catalase activity and total antioxidant capacity. MDA level in the liver was 0.33 ± 0.07 μM/mg protein which is significantly (p < 0.05) higher than that of the control group (0.17 ± 0.06 μM/mg protein), but in brain, there was no significant difference. PC-OOH level in the ethanol-treated group was 46.91 ± 12.87 pmol/mg in liver and 71.97 ± 26.12 pmol/mg protein in brain while PC-OOH level of control group was 21.40 ± 10.71 pmol/mg protein in liver and that in brain was 25.29 ± 5.67 pmol/mg protein pmol. Thus, PC-OOH levels in both liver and brain were significantly (p < 0.05) higher than that of control group. PC-OOH content in the liver and brain correlated significantly (p < 0.05) with catalase activity and total antioxidant capacity (TAC).

Conclusion: The study demonstrates that PC-OOH content in liver and brain tissues may be a marker for alcohol-induced oxidative stress.

Keywords: Alcohol Toxicity, Oxidative Stress, Phosphatidylcholine Hydroperoxide, Liver, Brain, Biomarker
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