VacA, CagA, IceA and OipA Genotype Status of Helicobacter pylori Isolated from Biopsy Samples from Iranian Dogs
Purpose: To investigate the state of vacA, cagA, oipA and iceA genotypes of H. pylori isolated from gastric biopsy samples of dogs.
Methods: A total of 240 gastric biopsy samples were taken from 240 dogs using gastric endoscope. All the samples were cultured and H. pylori-positive samples were analyzed for the presence of various genotypes using polymerase chain reaction (PCR).
Results: Thirteen out of 240 (5.41 %) samples were positive for H. pylori with a higher prevalence of the bacterium in dogs with gastric ulcer (6.36 %). The most commonly detected genotypes were vacA s1a (53.84 %), cagA (46.15 %) and vacA m2 (38.46 %). Total prevalence of iceA1, iceA2 and oipA genotypes were 23.07, 10.8 and 10.81 %, respectively. M2s1a (30.76 %), m1am2 (30.76 %) and m1as1a (23.07 %) had the highest prevalence among other detected combined genotypes. Twenty-two different genotypic combinations were detected with higher prevalence of s1a/cagA+/iceA1/oipA- (23.07 %), m2/cagA+/iceA1/oipA- (23.07 %), and s1b/cagA+/iceA1/oipA- (15.38 %) genotypes.
Conclusion: The presence of similar genotypes in H. pylori strains of gastric biopsy samples of dogs and those of human clinical specimens suggest that dogs may be the source of the bacterium. Proper handwashing and avoidance contact with feces and saliva of dogs are recommended to prevent transmission of H. pylori.
Keywords: Helicobacter pylori, Genotype, Genotyping, Gastric biopsy, Iranian dogs
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.