Determination of Phytochemical Compounds, and Tyrosinase Inhibitory and Antimicrobial Activities of Bioactive Compounds from Streblus ilicifolius (S Vidal) Corner
Purpose: To determine the phytochemical content, and tyrosinase inhibitory and antimicrobial activities of the wood from Streblus ilicifolius (S. Vidal) Corner
Methods: The dried wood of S. ilicifolius (8.70 kg) was extracted by maceration to give petroleum ether, ethyl acetate, ethanol and water extracts, respectively. Dopachrome method was used to determine antityrosinase activity. Agar disc diffusion and modified broth microdilution methods were used to determine antimicrobial activity. Chromatographic techniques were used for phytochemical investigation. The structures elucidation of isolated compounds were identified by physical properties and spectroscopic data including UV, IR, NMR and MS data and confirmed by comparison with previously reports.
Results: The ethanol extract exhibited tyrosinase inhibition and antimicrobial activity against the Grampositive bacteria, Staphylococcus epidermidis and S. aureus. Phytochemical investigation showed five compounds, namely, (E)-2,4-dihydroxy-3 (3,7-dimethyl-2,6-octadienyl) benzaldehyde (1), phydroxybenzoic acid methyl ester (2), umbelliferone (3), moracin M (4), trans-resveratrol (5). Compound 4 exhibited tyrosinase inhibition with half maximal inhibitory concentration (IC50) of 67.69 μg/ml, while compound 1 displayed strong activity against S. epidermidis, S. aureus and methicillin-resistant S. aureus (MRSA) with minimum inhibitory concentration (MIC) of 8, 4 and 8 μg/ml, respectively and minimum bactericidal concentration (MBC) of 32, 16 and 64 μg/ml, respectively.
Conclusion: This is the first report of the biological activities and phytochemical composition of S. ilicifolius and the results indicate the high potentials of the plant for commercial applications such as in facial whitening and anti-acne cream.
Keywords: Streblus ilicifolius, Moraceae, Tyrosinase inhibition, Antimicrobial, Anti-acne, Methicillinresistant Staphylococcus aureus
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.