Chromatographic-mass spectrometric analysis of ethanol extract of Maesa perlaria var formosana

  • Jyh-Ferng Yang
  • Ming-Tsai Liang
  • Cheng-Hong Yang
  • Zi-jie Gao
  • Yuh-Wern Wu
  • Li-Yeh Chuang
Keywords: Maesa perlaria var. formosana, 2, 4-Di-tert-butylphenol, Stigmasterol, Campesterol, Gas chromatography-mass spectrometry

Abstract

Purpose: This study analyzes the chemical composition of ethanol root extracts of Maesa perlaria var. formosana by gas chromatography-mass spectrometry (GC-MS).

Methods: The dried root of Maesa perlaria var. formosana was extracted with 95 % ethanol for composition analysis under the following optimum GC-MS conditions: 250 °C inlet temperature, 250 °C MSD detector temperature, and GC oven temperature programmed as follows: initial temperature held at 70 °C for 15 min, then increased at a rate of 2.5 °C/min and held at 170 °C for 15 min; then raised at a rate of 2 °C/min and kept at 180 °C for 20 min; then raised at 2 °C/min and kept at 250 °C for 20 min. Finally, it was raised at 3 °C/min and kept at 280 °C for 15 min.

Results: A total of 59 chemical compounds were identified, representing 88.82 % of the composition of the ethanol extracts. The three major components, include 2,4-di-tert-butylphenol (16.76 %), stigmasterol (15.86 %) and campesterol (7.33 %)

Conclusion: The results show that a total of 59 components were identified in the ethanol extract of Maesa perlaria var. formosana. The major component, 2,4-Di-tert-butylphenol, exhibits various biological activities.

Keywords: Maesa perlaria var. formosana, 2,4-Di-tert-butylphenol, Stigmasterol, Campesterol, Gas chromatography-mass spectrometry

Published
2016-05-30
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996