Protective effect of (-)-epigallocatechin gallate on ultraviolet b-induced skin damage in hairless mice

  • Chang Liu
  • Xin-Qiang Zheng
  • Li-Ping Xiang
  • Jian-Liang Lu
  • Curt Anthony Polito
  • Yue-Rong Liang
Keywords: UV-B, Green tea EGCg, Photoprotection, Stratum corneum, Mitochondrion, Melanosome

Abstract

Purpose: To investigate the protective effect of green tea (-)-epigallocatechin gallate (EGCg) on ultraviolet B (UV-B)-induced skin damages in hairless mice in order to develop a natural sunscreen compound for use in skin care products and cosmetics.
Methods: EGCg was dissolved in acetone at concentrations of 1.0, 10.0 and 50.0 mg/mL, and topically applied to the skin of hairless mice at doses of 0.2 mL/cm2, with acetone as control. The mice were then irradiated by 100 μW/cm2 UV-B for 30 min daily. EGCg treatment and UV-B irradiation were carried out daily for 28 consecutive days. The mice were then sacrificed and their dorsal skin examined by transmission electron microscopy (TEM) on the 28th day.
Results: UV-B irradiation induced severe macroscopic skin damage including chapping, cracking and abnormal desquamation in the treated hairless mice. EGCg showed dose-dependent protective effects against UV-B induced damage on the skin. Treatments with 10.0 and 50.0 mg/mL EGCg alleviated UVB- induced skin damage by suppressing both keratinocyte apoptosis and mitochondrial dysfunction, along with inhibiting the production of melanin pigment.
Conclusion: Topical application of green tea EGCg shows dose-dependent protective effect against UV-B-induced damage on hairless mouse skin. Thus, the plant compound can potentially be used as an alternative agent for photoprotection against UV-B exposure.

Keywords: UV-B, Green tea EGCg, Photoprotection, Stratum corneum,  Mitochondrion, Melanosome

Published
2016-07-11
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996