PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Effect of inulin supplementation in male mice fed with high fat diet on biochemical profile and α-amylase gene expression

Zahra Pouyamanesh, Mahsa M Amoli, Parichehreh Yaghmaei, Azadeh Ebrahim-Habibi

Abstract


Purpose: To evaluate the preventive and therapeutic effects of inulin  supplementation in Naval Medical Research Institute (NMRI) male mice fed with high fat diet.
Methods: NMRI male mice (n = 36) were divided into three groups. Control (C1), obese (O1) and experimental mice (E1) were fed during 8 weeks as follows: C1 with normal rodent pellet, O1 with high fat diet, and E1 with high fat diet plus 20 % inulin. C2, O2, and E2 were fed as follows: C2 with normal rodent pellets for 12 weeks; O2 with high fat diet during 8 weeks and switched to normal rodent pellet during next 4 weeks; and E2 with high fat diet over a period of 8 weeks and switched to normal rodent pellet plus 20 % inulin for 4 weeks. Body weight, serum glucose,  triglycerides, total cholesterol, high density lipoprotein (HDL), low density  lipoprotein (LDL), and hepatic α-amylase gene expression were measured.
Results: Groups receiving high fat diet showed higher weight (30.71 ± 0.66 g in O2, p < 0.001), nonfasting blood glucose levels (257.69 ± 5.10 mg/dl in O2, p < 0.001), TG (282.15 ± 1.83 mg/dl in O2, (p < 0.001)), and cholesterol levels  (335.72 ± 2.23 mg/dl in O2, (p < 0.001)), compared with control. In C2 group, mean body weight was 25.71 ± 0.54 g, non-fasting blood level 161.54 ± 4.48 mg/dl, TG level 214.29 ± 5.54 mg/dl, and cholesterol level 164.29 ±4.57 mg/dl. Compared to obese group, mice receiving inulin showed lower blood glucose levels (223.10 ± 8.7 mg/dl in E2, p < 0.001), body weight (27.86 ± 0.57 g in E2, p < 0.001), TG (232.14 ± 4.02 mg/dl in E2, p < 0.001) and cholesterol (249.97 ±
2.28 in E2, p < 0.001). A slight decrease in hepatic α-amylase gene expression was observed only in E1.
Conclusion: Besides its sweetening properties, inulin may also find use as a potential anti-obesity compound.


Keywords: High-fat diet, Inulin, Obesity, Blood glucose, Biochemical profile




http://dx.doi.org/10.4314/tjpr.v15i6.12
AJOL African Journals Online