A study of lectin activity in buds of Sophora japonica L
Purpose: To identify lectins in Sophora japonica L. (green flower buds, fully formed flower buds, and flower buds as they begin to open) and to study their activity.
Methods: Lectin activity was studied using rat hemagglutination method. The protein concentration of the extracts of the agglutinate was determined using Bradford assay.
Results: Lectin activity of green flower buds was 1.61 ± 0.11 units/mg protein; fully formed flower buds, 1.81 ± 0.08 units/mg protein; flower buds as they began to open, and 2.05 ± 0.05 units/mg protein. The protein content of extracts from the buds of Sophora japonica L. collected at the stage of green flower buds, at the stage of formed buds, and at the stage of opening flower buds were 3.97 ± 0,04, 3.53 ± 0.07 and 3.13 ± 0.09 mg/ml respectively.
Conclusion: This study shows the existence of lectins in Sophora japonica L. buds studied at three different stages of development. The highest lectin activity and protein content are found in the stage of green flower buds.
Keywords: Lectins, Sophora japonica L, Flower buds, Ratuserytroagglutination