Headspace solid-phase microextraction and gas chromatography–mass spectrometry of volatile components of Chrysanthemum morifolium Ramat

  • Yanbing Wu
  • Zhenmin Yan
  • Junjun Zhao
  • Yinghui Zhu
Keywords: Chrysanthemum morifolium Ramat., Headspace Solid-phase Microextraction, Gas Chromatography–Mass Spectrometry, Volatile component

Abstract

Purpose: To extract and analyze the volatile components of Chrysanthemum morifolium Ramat. 'huaiju' by headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC–MS).

Methods: Volatile components were extracted by HS-SPME and identified by GC–MS. The relative contents of the components were determined by area normalization.

Results: The enhanced SPME conditions of C. morifolium involved sample extraction using a 65 μm polydimethylsiloxane/divinylbenzene extraction fiber after balancing for 40 min at 80 °C. A total of 48 components of the essential oil were identified. The major constituents are 2,6,6-trimethylbicyclo[3.1.1]hept-2-en-4-ol, acetate (15.90 %), 4,6,6-trimethyl-bicyclo[3.1.1]hept-3-en-2-one (14.86 %), 2,7,7-trimethyl-bicyclo[3.1.1]hept-2-en-6-one (13.08 %), and cyclohexene,3-(1,5-dimethyl-4-hexenyl)-6- methylene (5.97 %).

Conclusion: HS-SPME and GC–MS are convenient, rapid, and reliable approaches for analyzing the volatile components of C. morifolium.

Keywords: Chrysanthemum morifolium Ramat., Headspace Solid-phase Microextraction, Gas Chromatography–Mass Spectrometry, Volatile component

Published
2016-11-14
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996