PROMOTING ACCESS TO AFRICAN RESEARCH

Tropical Journal of Pharmaceutical Research

Log in or Register to get access to full text downloads.

Remember me or Register



Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species

Alvi Milliana, A.S. Noorhamdani, Sri Poeranto, Kusworini Handono, Sumarno Reto Prawiro, Avin Ainur Fitrianingsih, Lailia Nur Rachma

Abstract


Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion factor and examine its ability to cross-react with the OMPs of other Shigella species.
Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili  using a pili bacterial cutter in a solution of 0.5 % n-octyl-β-D-glucopyranoside. Hemagglutination was evaluated using mice erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with  antibodies was examined by Western blotting,
Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species.
Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein, and antibodies against it can cross-react with the OMPs of other Shigella species.


Keywords: Shigella flexneri OMP, Outer membrane protein, Protein adhesion, Antibody, Cross-reaction




http://dx.doi.org/10.4314/tjpr.v16i2.2
AJOL African Journals Online