Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species

  • Alvi Milliana
  • A.S. Noorhamdani
  • Sri Poeranto
  • Kusworini Handono
  • Sumarno Reto Prawiro
  • Avin Ainur Fitrianingsih
  • Lailia Nur Rachma
Keywords: Shigella flexneri OMP, Outer membrane protein, Protein adhesion, Antibody, Cross-reaction

Abstract

Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion factor and examine its ability to cross-react with the OMPs of other Shigella species.
Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili  using a pili bacterial cutter in a solution of 0.5 % n-octyl-β-D-glucopyranoside. Hemagglutination was evaluated using mice erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with  antibodies was examined by Western blotting,
Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species.
Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein, and antibodies against it can cross-react with the OMPs of other Shigella species.


Keywords: Shigella flexneri OMP, Outer membrane protein, Protein adhesion, Antibody, Cross-reaction

Published
2017-03-06
Section
Articles

Journal Identifiers


eISSN: 1596-9827
print ISSN: 1596-5996