Anti-inflammatory and anti-apoptotic effect of Rhodiola crenulata extract on spinal cord injury in rats
Abstract
Purpose: To investigate the effects of Rhodiola crenulata on injured spinal cord tissue recovery due to the presence of neuroprotective constituents such as salidroside, tyrosol, rosavins, and crenulatanoside.
Methods: Effect of R. crenulata extract (RCE) at doses of 10, 20 and 50 mg kg-1 was investigated for spinal cord injury (SCI) recovery in rat. The anti-inflammatory activity was estimated by haematoxylineosin (H&E) staining and myeloperoxidase activity assay, while the anti-apoptotic effect was investigated by terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) and caspase-3 activity assays in SCI tissue.
Results: RCE (10 mg mL-1) treatment resulted in a Basso, Beattie and Bresnahan (BBB) score of 14.50 ± 1.22, while negative control showed a BBB score of 6.17 ± 1.60. H&E staining in test groups treated with 20 and 50 mg kg-1 RCE showed statistically different (p < 0.05) histological scores (2.0 ± 0.37 and 1.83 ± 0.31, respectively) which are higher than those of SCI (S)-induced group. The estimates of apoptosis in SCI tissue) revealed significant differences from the normalised levels in RCE-treated groups.
Conclusion: The plant extract produced good recovery of injured spinal tissue, suggesting that further clinical trials and chemical constituent studies are warranted.
Keywords: Rhodiola crenulata, Haematoxylin-eosin, Caspase-3, Anti-inflammatory, Anti-apoptotic
Submission of a manuscript to this journal is a representation that the manuscript has not been published previously and is not under consideration for publication elsewhere.
All authors named in each manuscript would be required to sign a form (to be supplied by the Editor) so that they may retain their copyright in the article but to assign to us (the Publishers) and its licensees in perpetuity, in all forms, formats and media (whether known or created in the future) to (i) publish, reproduce, distribute, display and store the contribution, (ii) translate the contribution into other languages, create adaptations, reprints, include within collections and create summaries, extracts and/or abstracts of the contribution, (iii) create any other derivative works(s) based on the contribution, (iv) to exploit all subsidiary rights in the contribution, (v) the inclusion of electronic links from the contribution to third party material where-ever it may be located, and (vi) license any thrid party to do any or all of the above.